Optimization research on preparation conditions of polymer-shelled microbubble contrast agents

doi:10.3969/j.issn.2095-4344.2014.30.016

Abstract

Abstract:

BACKGROUND: In previous experiments, a polymer-shelled microbubble contrast agent was successfully prepared using L-polylactic acid-polyethylene glycol methyl-covered liquid fluorocarbon perfluoropentane, which developed well in vivo and in vitro under a second harmonic contrast-enhanced ultrasound with a low mechanical index
OBJECTIVE: To optimize the conditions for preparing polymer-shelled microbubble contrast agents with high yield and suitable size.
METHODS: The polymer-shelled microbubble contrast agent containing perfluoropentane was prepared by single emulsion method. Three preparation conditions, including polymer mass/perfluoropentane volume ratio (4/1, 2/1, 1/1, 1/2), hemogenate speed (18 000, 26 000, 35 000 r/min) and time (15, 30, 60, 120 seconds), were optimized, and the microbubble yield and size were set as judge standard. The contrast-enhanced ultrasonography of rabbit kidney was performed using the optimized microbubbles, and the arrival time, time to peak, half time of descending and peak intensity were analyzed by TCA software.
RESULTS AND CONCLUSION: The preparation conditions were optimized as a polymer mass/perfluoropentane volume ratio of 2/1, homogenate speed of 26 000 r/min and homogenate time of 60 seconds, which presented a high microbubble yield of (1.8±0.4)×10⁹/mL and mean size of (3.7±1.3) μm with uniform distribution. The arrival time, time to peak, half time of descending and peak intensity were detected as (3.1±0.6) seconds, (2.9±0.5) seconds, (4.0±0.7) seconds and (4.7±1.1)×10^-5 AU, respectively. The polymeric microbubbles prepared by optimized conditions were of high yield, with suitable size and good contrast-enhanced effect in vivo.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: constrast media, ulatrsonography, polymers

CLC Number:

R318

Huang Hong-jie, Lin Peng, Huang Rong-sen, Lin Yi-kai, Su Jie, Chen Wan-ping, Lin Li-wu, Xue En-sheng, Chen Zhi-kui . Optimization research on preparation conditions of polymer-shelled microbubble contrast agents[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(30): 4857-4862.

Figures/Tables 4

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